| Solution Information | help | |
| Enzyme: | Protein Rev [8-24] | |
| inhibitor: | BDBM44551 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine dose response curves for 28 available out of 28 test compounds that confirmed activity by Relative FRET and Acceptor channel criteria in a Confirmation screen (PubChem AID = 837) which followed a primary screen entitled, "Primary biochemical high-throughput screening assay for inhibitors of the HIV-REV RRE RNA interaction (disruption of protein-RNA interaction)" (PubChem AID = 704). As with the primary and confirmation screens, this assay is based on the ability of the Rev peptide to form a complex with the Rev-Responsive Element (RRE) of double-stranded viral mRNA. Both binding partners have been labeled with appropriate fluorophores in order to monitor their interaction by fluorescence resonance energy transfer (FRET). A Cy5-labeled RNA target harboring the Rev Response Element (Cy5-RRE) is first incubated with test compounds, followed by addition of a Fluorescein-labeled Rev peptide (Fl-Rev) and reading of the FRET flu | |
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